Modification History
Not applicable.
Unit Descriptor
Unit descriptor |
This unit of competency covers the ability to isolate, purify, verify and manipulate biomolecules and their products. This work requires close attention to working with small volumes, multiple-step procedures and prevention of contamination. Personnel are required to apply a wide range of molecular biology tests and procedures. |
Application of the Unit
Application of the unit |
This unit of competency is applicable to technical officers working in manufacturing (e.g. macro, micro, nanotechnology, pharmaceutical and blood product), food processing, biomedical (e.g. forensics, pathology and veterinary) and environmental industry sectors. All operations must comply with relevant regulations, codes of practice, standards, test methods and enterprise procedures. Results are generally interpreted and reported to supervising scientists, medical, veterinary or other responsible officers of an enterprise, regulatory authority or legal agency. Industry representatives have provided case studies to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. These can be found at the end of this unit of competency under the section 'This competency in practice'. |
Licensing/Regulatory Information
Not applicable.
Pre-Requisites
Prerequisite units |
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MSL974006A |
Perform biological procedures |
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MSL973007A |
Perform microscopic examination |
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MSL973004A |
Perform aseptic techniques |
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Employability Skills Information
Employability skills |
This unit contains employability skills. |
Elements and Performance Criteria Pre-Content
Elements describe the essential outcomes of a unit of competency. |
Performance criteria describe the performance needed to demonstrate achievement of the element. Where bold italicised text is used, further information is detailed in the required skills and knowledge section and the range statement. Assessment of performance is to be consistent with the evidence guide. |
Elements and Performance Criteria
ELEMENT |
PERFORMANCE CRITERIA |
1. Interpret and schedule test requirements |
1.1. Review test request to identify samples to be tested, test method and equipment/instruments involved 1.2. Identify hazards and enterprise control measures associated with the sample, preparation methods, reagents and/or equipment |
2. Receive and handle samples |
2.1. Log and label samples according to enterprise procedures 2.2. Record sample description, compare with specification and note and report discrepancies 2.3. Store samples in accordance with enterprise and test method requirements 2.4. Maintain chain of custody, traceable to the worker, for all samples |
3. Prepare equipment and reagents |
3.1. Set up equipment/instrumentation in accordance with test method requirements and perform pre-use and safety checks 3.2. Select and collect reagents in accordance with test method requirements 3.3. Prepare and label reagents in accordance with test method requirements |
4. Extract, verify and manipulate biomolecules |
4.1. Produce/extract biomolecules from samples using appropriate isolation methods 4.2. Prevent contamination of samples by unwanted biomolecules 4.3. Recognise the presence of common inhibitors of biomolecular reactions and take corrective action 4.4. Quantify and qualify biomolecular yields from purified extractions 4.5. Use appropriate techniques to prepare and test a range of biomolecular samples 4.6. Use controls and reference standards to confirm the integrity of biomolecular sample preparation and procedures |
5. Process data |
5.1. Record test data noting atypical observations 5.2. Ensure results are consistent with reference standards and expectations 5.3. Record and report results in accordance with test methods 5.4. Interpret trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel 5.5. Troubleshoot basic procedure, reagent or equipment problems which have led to atypical data or results |
6. Maintain a safe work environment |
6.1. Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel 6.2. Minimise the generation of wastes 6.3. Ensure the safe disposal of wastes, including hazardous wastes and tested samples 6.4. Clean, care for and store equipment and reagents |
7. Report and communicate results |
7.1. Record approved data into enterprise system 7.2. Keep accurate, traceable work records to protect the enterprise's intellectual property rights 7.3. Maintain confidentiality and security of enterprise information and laboratory data 7.4. Maintain equipment logs in accordance with enterprise procedures |
Required Skills and Knowledge
REQUIRED SKILLS AND KNOWLEDGE |
This section describes the skills and knowledge required for this unit. |
Required skills |
Required skills include:
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Required knowledge |
Required knowledge includes:
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Evidence Guide
EVIDENCE GUIDE |
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The Evidence Guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package. |
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Overview of assessment |
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Critical aspects for assessment and evidence required to demonstrate competency in this unit |
Assessors should ensure that candidates can:
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Context of and specific resources for assessment |
This unit of competency is to be assessed in the workplace or simulated workplace environment. This unit of competency may be assessed with:
Resources may include:
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Method of assessment |
The following assessment methods are suggested:
In all cases, practical assessment should be supported by questions to assess underpinning knowledge and those aspects of competency which are difficult to assess directly. Where applicable, reasonable adjustment must be made to work environments and training situations to accommodate ethnicity, age, gender, demographics and disability. Access must be provided to appropriate learning and/or assessment support when required. The language, literacy and numeracy demands of assessment should not be greater than those required to undertake the unit of competency in a work like environment. |
This competency in practice |
Industry representatives have provided the case studies below to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. Biomedical As part of a diagnostic service to verify progenitor status of livestock, a technician is required to extract DNA from a blood sample, perform the PCR to amplify micro-satellite DNA and prepare the sample for DNA electrophoresis and fragment size analysis. The technician provides documentation to meet evidentiary standards. The technician understands the implications of the tests for the client and is careful to ensure the sample can be traced from the source, that no contamination takes place and that the results are kept confidential. Food processing A meat export company has commissioned a study of the effectiveness of the introduction of a 'cold-chain' process to a client country. The company requires rapid results. As part of the monitoring team, a technician is required to perform routine testing of surface swabs of meat samples for bacterial contamination using a PCR analytic technique. Although the tests are quite routine, the technician pays close attention to all aspects of the work as the consequences of invalid results would be severe for the company and laboratory. The technician also keeps comprehensive work records and maintains strict confidentiality. |
Range Statement
RANGE STATEMENT |
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The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included. |
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Codes of practice |
Where reference is made to industry codes of practice, and/or Australian/international standards, it is expected the latest version will be used |
Standards , codes , procedures and /or enterprise requirements |
Standards, codes, procedures and/or enterprise requirements may include:
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Hazards |
Hazards may include:
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Safe work practices and hazard control measures |
Safe work practices and hazard control measures may include:
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Equipment and instrumentation |
Equipment and instrumentation may include:
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Reagents |
Reagents may include:
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Molecular biology tests and procedures |
Molecular biology tests and procedures may include:
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Corrective action |
Corrective action may involve:
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Records |
Records may include:
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Occupational health and safety (OHS ) and environmental management requirements |
OHS and environmental management requirements:
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Unit Sector(s)
Unit sector |
Testing |
Competency field
Competency field |
Co-requisite units
Co-requisite units |
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